ABSTRACT
Objective: To study the law of quality value transmitting of Artemisiae Scopariae Herba (ASH) standard decoction. Methods: Fifteen batches of ASH standard decoction were prepared. Fingerprints of these 15 batches standard decoction and its raw pieces were determined by HPLC, the control fingerprints were established, the common peaks were calibrated and the similarity was evaluated. Components of common peaks were identified by Q-TOF/MS and the contents of components confirmed by reference substance were determined. The common peaks transfer number, peak area ratio, index components transfer rate and extraction rate were used to analyze the quality value transfer rule of standard decoction. Results: The similarity of fingerprints of ASH and its standard decoction were greater than 0.9, 16 and 15 common peaks were calibrated respectively, and the transfer rate of the common peaks number was 93.75%. Thirteen components of the common peaks were confirmed by reference substance, including 1-caffeoylquinic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-hydroxyacetophenone, 1,3-dicaffeoylquinic acid, rutin, hyperoside, isoquercetin, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid. Caffeoylquinic acid glucoside and a di-C-glycoside named apigenin 6,8-di-C-β-D-glucoside were first detected in ASH. The ratio of the common peaks area of ASH standard decoction to that of ASH raw pieces showed a good positive linear relationship with the components transfer rate. The average transfer rates of neochlorogenic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were more than 100%, while the average transfer rates of chlorogenic acid and 3,5-dicaffeoylquinic acid were only 47.59% and 22.33% respectively, suggesting that the organic acid components may be transformed into each other during the preparation of standard decoction, and part of chlorogenic acid may be transformed into cryptochlorogenic acid and neochlorogenic acid; 3,5-dicaffeoylquinic acid may be partially converted into 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid. The average transfer rates of rutin and hyperoside were 31.36% and 28.36%, respectively, and that of other components were between 50% and 70%. The average extraction rate of standard decoction was 19.76%. Conclusion: The laws of quality value transmitting of ASH standard decoction revealed in this study laid a foundation for the establishment of the material reference of ASH formula granules and the classic prescriptions containing ASH.
ABSTRACT
The main chemical components of Artemisiae Scopariae Herba (ASH) include coumarins, flavonoids, organic acids, essential oils, and so on. Except for the traditional actions of clearing and draining dampness-heat, and disinhibiting gallbladder and anti-icteric, ASH has multiple pharmacological activities, such as antipyretic, analgesic, anti-inflammatory, antiviral, antitumor, hypotensive, hypolipidemic, anti-osteoporotic, neuroprotective, metabolic regulation effects, as well as prevention of Alzheimer’s disease, whose mechanism of actions are complex. This article reviews pharmacological actions and the corresponding mechanism of ASH, which can provide reference for the research, development and clinical application of ASH and its preparations.
ABSTRACT
Chemical profiling of a given herbal medicine( HM) is the prerequisite for clarifying the effective material basis and therapeutic mechanisms,and it is an important integral part of traditional Chinese medicine chemical biology( TCMCB). In current study,we aimed to propose a new strategy for fast chemical characterization of HM by using reversed phase liquid chromatography-hydrophilic interaction chromatography-predictive multiple reaction monitoring( RPLC-HILIC-p MRM),and Artemisiae Scopariae Herba was employed in this study to illustrate the entire strategy. In response to wide polarity spanning of the diverse chemical clusters in Artemisiae Scopariae Herba,RPLC and HILIC were coupled in series to retain and separate hydrophilic and hydrophobic components simultaneously by identifying the characteristics of chromatographic separation. Most of the chemical constituents in traditional Chinese medicine can be predicted by summarizing the results of chemical constituents of the same genera and introducing primary metabolites and possible substitution reaction types. Therefore,we constructed predictive ion pairs to rapidly identify the chemical constituents of Artemisiae Scopariae Herba. After comparison with control products,discussion on fragmentation pattern,and access to relevant information from literature and databases,a total of 139 components were detected and structurally annotated by matching the obtained spectral data with the information of authentic compounds. Above all,RPLC-HILIC-p MRM could be used as an eligible analytical tool for the chemical profiling of HMs.
Subject(s)
Artemisia , Chemistry , Chromatography, Reverse-Phase , Hydrophobic and Hydrophilic Interactions , Medicine, Chinese Traditional , Plants, Medicinal , ChemistryABSTRACT
OBJECTIVE: To establish the fingerprint of the serum containing drug of Yinchenhao Decoction (YCHD), and analyze the drug-originated constituents in serum. METHODS: An HPLC method was set up on a Symmetry C18(4.6 mm × 250 mm, 5 μm) column with a Security Guard Cartridges C18 (4.0 mm × 3.0 mm) guard column by gradient elution of acetonitrile-0.1% phosphoric acid in water at the flow rate of 1.0 mL · min-1. The column temperature was maintained at 30℃. The detection was carried out at 238 and 440 nm. SD rats were taken as serum donors. Ten batches of serum containing drugs were prepared after ig administration of YCHD. The fingerprints of these serum samples were determined, the common modes were established, and the similarities between fingerprints were evaluated. Drug-originated constituents in blood were distinguished by comparing the fingerprint of serum containing YCHD with the one of control serum. The sources of drug-originated constituents were analyzed by comparing the fingerprint of serum containing YCHD with the ones of serum containing individual herbs of YCHD. Drug-originated constituents in blood were identified by comparing the retention time and UV spectra of peaks in fingerprint of serum containing YCHD with the ones of YCHD and reference substances, respectively. RESULTS: The similarities between the fingerprints of ten batches of serum containing YCHD and common models at 238 and 440 nm were greater than 0.904 and 0.903, respectively. Twenty common peaks of drug-originated constituents were identified in the fingerprints of serum containing drug of YCHD at 238 and 440 nm. Out of them, six were the original compounds, the other 14 were metabolites. Three constituents out of six original form ones were identified as geniposidic acid and genipo-side (iridoids originated from GF), and rhein (anthraquinone from RRR), and the other three were identified as an anthraquinone from RRR and two crocetin derivatives from FG. Out of 14 metabolites, one was identified as the metabolite of iridoid, four were from anthraquinone, and nine were from crocetin derivatives. CONCLUSION: The established method is accurate, reliable and can be used for the analysis of drug-originated constituents in serum containing drug of YCHD.
ABSTRACT
Objective: To research the influence of prescription compatibility on the extraction rate of main constituents in Yinchenhao Decoction (YCHD). Methods: The extraction volume of two iridoids (geniposide and deacetylasperulosidic acid methyl ester), two crocetin derivatives (crocin I and crocin II), three bound anthraquinones (aloe-emodin-8-O-glucoside, chrysophanol-1-glucoside, and emodin-8-glucoside), five free anthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion), two tannins (gallic acid and catechin), and chlorogenic acid in Artemisiae Scopariae Herba (ASH), Gardeniae Fructus (GF), Rhei Radix et Rhizoma (RRR), the compatibility of ASH and GF (compatibility A), the compatibility of ASH and RRR (compatibility B), the compatibility of GF and RRR (compatibility C), and the compatibility of ASH, GF, and RRR (compatibility D) were determined by HPLC. Taking the extraction volume in single medicine as 100%, the extraction rates of 15 constituents mentioned above in compatibilities A-D were calculated. Results: The extraction rates of geniposide, crocin I, and crocin II in compatibilities A, C, and D, deacetylasperulosidic acid methyl ester in compatibility C, gallic acid in compatibilities C and D, and chlorogenic acid in compatibilities A-D decreased. The extraction rates of deacetylasperulosidic acid methyl ester in compatibilities A and D, three bound anthraquinones, aloe-emodin, and chrysophanol in compatibility B, chrysophanol and physcion in compatibilities C and D increased. Conclusion: Prescription compatibility has some influence on the extraction rate of main constituents in YCHD.